oh2vsj6v

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?:abstract	BACKGROUND: Schistosomiasis is a chronic, debilitating infectious disease caused by members of the genus Schistosoma. Previous findings have suggested a relationship between infection with Schistosoma spp. and alterations in the liver and spleen of infected animals. Recent reports have shown the regulatory role of noncoding RNAs, such as long noncoding RNAs (lncRNAs), in different biological processes. However, little is known about the role of lncRNAs in the mouse liver and spleen during Schistosoma japonicum infection. METHODS: In this study, we identified and investigated lncRNAs using standard RNA sequencing (RNA-Seq). The biological functions of the altered expression of lncRNAs and their target genes were predicted using bioinformatics. Ten dysregulated lncRNAs were selected randomly and validated in reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) experiments. RESULTS: Our study identified 29,845 and 33,788 lncRNAs from the liver and spleen, respectively, of which 212 were novel lncRNAs. We observed that 759 and 789 of the lncRNAs were differentially expressed in the respective organs. The RT-qPCR results correlated well with the sequencing data. In the liver, 657 differentially expressed lncRNAs were predicted to target 2548 protein-coding genes, whereas in the spleen 660 differentially expressed lncRNAs were predicted to target 2673 protein-coding genes. Moreover, functional annotation showed that the target genes of the differentially expressed lncRNAs were associated with cellular processes, metabolic processes, and binding, and were significantly enriched in metabolic pathways, the cell cycle, ubiquitin-mediated proteolysis, and pathways in cancer. CONCLUSIONS: Our study showed that numerous lncRNAs were differentially expressed in S. japonicum-infected liver and spleen compared to control liver and spleen; this suggested that lncRNAs may be involved in pathogenesis in the liver and spleen during S. japonicum infection. [Image: see text]
is ?:annotates of	<https://research.tib.eu/covid-19/entity/oh2vsj6v_hasAnnotation_C0006826> <https://research.tib.eu/covid-19/entity/oh2vsj6v_hasAnnotation_C0074992> <https://research.tib.eu/covid-19/entity/oh2vsj6v_hasAnnotation_C0162327> <https://research.tib.eu/covid-19/entity/oh2vsj6v_hasAnnotation_C0887909> <https://research.tib.eu/covid-19/entity/oh2vsj6v_hasAnnotation_C1332838> <https://research.tib.eu/covid-19/entity/oh2vsj6v_hasAnnotation_C3839127>
?:creator	<https://research.tib.eu/covid-19/entity/Xia%2C_Tianqi%3B_Giri%2C_Bikash_Ranjan%3B_Liu%2C_Jingyi%3B_Du%2C_Pengfei%3B_Li%2C_Xue%3B_Li%2C_Xuxin%3B_Li%2C_Shun%3B_Cheng%2C_Guofeng>
?:doi	10.1186/s13071-020-04457-9
?:doi	<https://research.tib.eu/covid-19/entity/10.1186/s13071-020-04457-9>
?:externalLink	<https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705434/>
?:journal	Parasit_Vectors
?:license	cc-by
?:pdf_json_files	document_parses/pdf_json/019d5fbb38f7f83cfe17dba5637d21ba91b56161.json
?:pmc_json_files	document_parses/pmc_json/PMC7705434.xml.json
?:pmcid	<https://research.tib.eu/covid-19/entity/PMC7705434>
?:publication_isRelatedTo_Disease	<https://research.tib.eu/covid-19/entity/COVID-19>
is ?:relation_isRelatedTo_publication of	<https://research.tib.eu/covid-19/entity/oh2vsj6v_HAS_C1332838_INTERACTS_WITH_C3494264> <https://research.tib.eu/covid-19/entity/oh2vsj6v_HAS_C3494264_ASSOCIATED_WITH_C0036329> <https://research.tib.eu/covid-19/entity/oh2vsj6v_HAS_C3494264_INTERACTS_WITH_C3839127>
?:sha_id	<https://research.tib.eu/covid-19/entity/019d5fbb38f7f83cfe17dba5637d21ba91b56161>
?:source	PMC
?:title	RNA sequencing analysis of altered expression of long noncoding RNAs associated with Schistosoma japonicum infection in the murine liver and spleen
?:type	<https://research.tib.eu/covid-19/vocab/Publication>
?:year	2020-12-01

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Value

?:abstract

BACKGROUND: Schistosomiasis is a chronic, debilitating infectious disease caused by members of the genus Schistosoma. Previous findings have suggested a relationship between infection with Schistosoma spp. and alterations in the liver and spleen of infected animals. Recent reports have shown the regulatory role of noncoding RNAs, such as long noncoding RNAs (lncRNAs), in different biological processes. However, little is known about the role of lncRNAs in the mouse liver and spleen during Schistosoma japonicum infection. METHODS: In this study, we identified and investigated lncRNAs using standard RNA sequencing (RNA-Seq). The biological functions of the altered expression of lncRNAs and their target genes were predicted using bioinformatics. Ten dysregulated lncRNAs were selected randomly and validated in reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) experiments. RESULTS: Our study identified 29,845 and 33,788 lncRNAs from the liver and spleen, respectively, of which 212 were novel lncRNAs. We observed that 759 and 789 of the lncRNAs were differentially expressed in the respective organs. The RT-qPCR results correlated well with the sequencing data. In the liver, 657 differentially expressed lncRNAs were predicted to target 2548 protein-coding genes, whereas in the spleen 660 differentially expressed lncRNAs were predicted to target 2673 protein-coding genes. Moreover, functional annotation showed that the target genes of the differentially expressed lncRNAs were associated with cellular processes, metabolic processes, and binding, and were significantly enriched in metabolic pathways, the cell cycle, ubiquitin-mediated proteolysis, and pathways in cancer. CONCLUSIONS: Our study showed that numerous lncRNAs were differentially expressed in S. japonicum-infected liver and spleen compared to control liver and spleen; this suggested that lncRNAs may be involved in pathogenesis in the liver and spleen during S. japonicum infection. [Image: see text]

is ?:annotates of