| Property | Value |
|---|
|
?:abstract
|
-
Objective: To quantify the levels of circulating exosomes derived from T-cells and monocytes and their possible associations with leukocyte subpopulations and cytokine milieu in Systemic Lupus Erythematosus (SLE). Methods: Total circulating exosomes (CD9(+)-Ex) and those derived from T-cells (CD3(+)-Ex) and monocytes (CD14(+)-Ex) were quantified by flow cytometry in 82 SLE patients and 32 controls. Leukocyte subsets and serum cytokines were analyzed by flow cytometry or by immunoassays. IFN-score was evaluated by real time RT-PCR in whole blood samples from a subgroup of 73 patients and 24 controls. Results: Activation markers (IFNR1 and BLyS) on monocytes, neutrophils and B-cells correlated inversely with circulating exosomes (CD9(+)-Ex, CD3(+)-Ex, and CD14(+)-Ex) in controls but directly with CD3(+)-Ex in patients (all p < 0.05). Although CD9(+)-Ex were increased in SLE, no differences were found in CD3(+)-Ex, supporting that exosome content accounts for this opposite role. Interestingly, CD4(+)CD28(null) cells correlated with CD3(+)-Ex in patients and controls, and displayed similar associations with leukocyte subsets in both groups. Additionally, CD3(+)-Ex correlated in patients with the expression of CD25 in CD4(+)CD28(null) cells. Furthermore, the activated status of this senescent subset was related to IFNα serum levels in controls and to IFN-score in SLE patients. Finally, patients presenting high IFN-score, in addition to elevated CD25(+)CD28(null) cells associated with the activation of myeloid cells, displayed higher levels of inflammatory cytokines and chemokines. Conclusion: Our results support a relationship between T-cell exosomes and cellular subsets in SLE according to type I IFN-signaling, which could amplify chronic immune activation and excessive cytokine/chemokine response.
|
|
is
?:annotates
of
|
|
|
?:creator
|
|
|
?:doi
|
|
|
?:doi
|
|
|
?:externalLink
|
|
|
?:journal
|
|
|
?:license
|
|
|
?:pdf_json_files
|
-
document_parses/pdf_json/164f9129403270018dc61c4aa2b598b289973133.json
|
|
?:pmc_json_files
|
-
document_parses/pmc_json/PMC7734125.xml.json
|
|
?:pmcid
|
|
|
?:publication_isRelatedTo_Disease
|
|
|
is
?:relation_isRelatedTo_publication
of
|
|
|
?:sha_id
|
|
|
?:source
|
|
|
?:title
|
-
Relationship Between T-Cell Exosomes and Cellular Subsets in SLE According to Type I IFN-Signaling
|
|
?:type
|
|
|
?:year
|
|
![[This page as RDF]](https://research.tib.eu/covid-19/static/rdf-icon.gif){kind=icon}