rpekzskg

Property	Value
?:abstract	OBJECTIVES: To examine whether specific T-cell-responses to SARS-CoV-2 peptides can be detected in COVID-19 using a whole-blood experimental setting, which may be further explored as a potential diagnostic tool. METHODS: We evaluated interferon (IFN)-Î³ levels after stimulating whole-blood with spike and remainder-antigens peptides megapools (MP) derived from SARS-CoV-2 sequences; interleukin (IL)-1ß, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17A, eotaxin, basic fibroblast growth factor (FGF), granulocyte-colony stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), IFN-Î³, Interferon gamma-induced protein 10 (IP-10), monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein (MIP)-1α, MIP-1ß, Platelet-derived growth factor (PDGF), RANTES (regulated on activation, normal T cell expressed and secreted), tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF) were also evaluated. RESULTS: IFN-Î³-response to spike and remainder-antigens MPs was significantly increased in 35 COVID-19 patients compared with 29 \'no COVID-19\' individuals (medians spike-MP: 0.26 vs 0, p = 0.0002; medians remainder-antigens-MP: 0.07 vs 0.02; p = 0.02). This response was detected independently of patients\' clinical parameters. IFN-Î³-response to SARS-CoV-2-unrelated antigens cytomegalovirus (CMV) and Staphylococcal Enterotoxin B (SEB) was similar in COVID-19 compared with \'no COVID-19\' individuals (median CMV: 3.46 vs 5.28, p = 0.16; median SEB: 12.68 vs 15.05; p = 0.1). In response to spike-MPs in COVID-19- compared with \'no COVID-19\' -individuals, we found significant higher median of IL-2 (50.08 vs 0, p = 0.0018), IFN-Î³ (90.16 vs 0, p = 0.01), IL-4 (0.52 vs 0, p = 0.03), IL-13 (0.84 vs 0, p = 0.007) and MCP-1 (4602 vs 359.2, p = 0.05). CONCLUSIONS: Immune response to SARS-CoV-2 peptides in a whole-blood assay is associated with COVID-19 and it is characterized by both Th1 and Th2 profile. This experimental approach may be useful for developing new T-cell based diagnostic tests for disease and vaccine settings.
is ?:annotates of	<https://research.tib.eu/covid-19/entity/rpekzskg_hasAnnotation_C0010825> <https://research.tib.eu/covid-19/entity/rpekzskg_hasAnnotation_C0162326> <https://research.tib.eu/covid-19/entity/rpekzskg_hasAnnotation_C1423842> <https://research.tib.eu/covid-19/entity/rpekzskg_hasAnnotation_C5203676>
?:creator	<https://research.tib.eu/covid-19/entity/Petrone%2C_Linda%3B_Petruccioli%2C_Elisa%3B_Vanini%2C_Valentina%3B_Cuzzi%2C_Gilda%3B_Najafi_Fard%2C_Saeid%3B_Alonzi%2C_Tonino%3B_Castilletti%2C_Concetta%3B_Palmieri%2C_Fabrizio%3B_Gualano%2C_Gina%3B_Vittozzi%2C_Pietro%3B_Nicastri%2C_Emanuele%3B_Lepore%2C_Luciana%3B_Antinori%2C_Andrea%3B_Vergori%2C_Alessandra%3B_Caccamo%2C_Nadia%3B_Cantini%2C_Fabrizio%3B_Girardi%2C_Enrico%3B_Ippolito%2C_Giuseppe%3B_Grifoni%2C_Alba%3B_Goletti%2C_Delia>
?:journal	Clin._microbiol._infect
?:license	unk
?:publication_isRelatedTo_Disease	<https://research.tib.eu/covid-19/entity/COVID-19>
is ?:relation_isRelatedTo_publication of	<https://research.tib.eu/covid-19/entity/rpekzskg_HAS_C0021740_STIMULATES_C0033684> <https://research.tib.eu/covid-19/entity/rpekzskg_HAS_C0030956_ASSOCIATED_WITH_C5203670> <https://research.tib.eu/covid-19/entity/rpekzskg_HAS_C0059386_ASSOCIATED_WITH_C5203670> <https://research.tib.eu/covid-19/entity/rpekzskg_HAS_C1423842_COEXISTS_WITH_C0030956>
?:source	WHO
?:title	A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients
?:type	<https://research.tib.eu/covid-19/vocab/Publication>
?:who_covidence_id	#845619
?:year	2020

Property

Value

?:abstract

OBJECTIVES: To examine whether specific T-cell-responses to SARS-CoV-2 peptides can be detected in COVID-19 using a whole-blood experimental setting, which may be further explored as a potential diagnostic tool. METHODS: We evaluated interferon (IFN)-Î³ levels after stimulating whole-blood with spike and remainder-antigens peptides megapools (MP) derived from SARS-CoV-2 sequences; interleukin (IL)-1ß, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17A, eotaxin, basic fibroblast growth factor (FGF), granulocyte-colony stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), IFN-Î³, Interferon gamma-induced protein 10 (IP-10), monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein (MIP)-1α, MIP-1ß, Platelet-derived growth factor (PDGF), RANTES (regulated on activation, normal T cell expressed and secreted), tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF) were also evaluated. RESULTS: IFN-Î³-response to spike and remainder-antigens MPs was significantly increased in 35 COVID-19 patients compared with 29 \'no COVID-19\' individuals (medians spike-MP: 0.26 vs 0, p = 0.0002; medians remainder-antigens-MP: 0.07 vs 0.02; p = 0.02). This response was detected independently of patients\' clinical parameters. IFN-Î³-response to SARS-CoV-2-unrelated antigens cytomegalovirus (CMV) and Staphylococcal Enterotoxin B (SEB) was similar in COVID-19 compared with \'no COVID-19\' individuals (median CMV: 3.46 vs 5.28, p = 0.16; median SEB: 12.68 vs 15.05; p = 0.1). In response to spike-MPs in COVID-19- compared with \'no COVID-19\' -individuals, we found significant higher median of IL-2 (50.08 vs 0, p = 0.0018), IFN-Î³ (90.16 vs 0, p = 0.01), IL-4 (0.52 vs 0, p = 0.03), IL-13 (0.84 vs 0, p = 0.007) and MCP-1 (4602 vs 359.2, p = 0.05). CONCLUSIONS: Immune response to SARS-CoV-2 peptides in a whole-blood assay is associated with COVID-19 and it is characterized by both Th1 and Th2 profile. This experimental approach may be useful for developing new T-cell based diagnostic tests for disease and vaccine settings.

is ?:annotates of