?:abstract
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Bats are natural reservoirs for potential zoonotic viruses In this study, next-generation sequencing was performed to obtain entire genome sequences of picornavirus from a picornavirus-positive bat feces sample (16BF77) and to explore novel viruses in a pooled bat sample (16BP) from samples collected in South Korea, 2016 Fourteen mammalian viral sequences were identified from 16BF77 and 29 from 16BP, and verified by RT-PCR The most abundant virus in 16BF77 was picornavirus Highly variable picornavirus sequences encoding 3D(pol) were classified into genera Kobuvirus, Shanbavirus, and an unassigned group within the family Picornaviridae Amino acid differences between these partial 3D(pol) sequences were ≥ 65 7% Results showed that one bat was co-infected by picornaviruses of more than two genera Retrovirus, coronavirus, and rotavirus A sequences also were found in the BP sample The retrovirus and coronavirus genomes were identified in nine and eight bats, respectively Korean bat retroviruses and coronavirus demonstrated strong genetic relationships with a Chinese bat retrovirus (RfRV) and coronavirus (HKU5-1), respectively A co-infection was identified in one bat with a retrovirus and a coronavirus Our results indicate that Korean bats were multiply infected by several mammal viruses
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