PropertyValue
?:abstract
  • Comprehensive libraries of plasmids for SARS-CoV-2 proteins with various tags (e.g. Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein-protein interactions between the SARS-CoV-2 virus and host proteins. To facilitate further cellular investigations, notably by imaging techniques, we present here a large library of SARS CoV-2 protein constructs fused with green and red fluorescent proteins and their initial characterization in various human cell lines including lung epithelial cell models (A549, BEAS-2B), as well as in budding yeast. The localization of a few SARS-CoV-2 proteins matches their proposed interactions with host proteins. These include the localization of Nsp13 to the centrosome, Orf3a to late endosomes, and Orf9b to mitochondria.
is ?:annotates of
?:creator
?:doi
?:doi
  • 10.1101/2020.12.19.423586
?:externalLink
?:license
  • unk
?:publication_isRelatedTo_Disease
?:source
  • WHO
?:title
  • A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterization in different cell types
?:type
?:who_covidence_id
  • #423586
?:year
  • 2020

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