yrgpy3zi

Property	Value
?:abstract	SESSION TITLE: Global Case Report Posters SESSION TYPE: Global Case Reports PRESENTED ON: October 18-21, 2020 INTRODUCTION: On December 2019 in Wuhan China there were a cluster of cases of an unknown etiology pneumonia, that we later discovered it was caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) [1] In May 2020, in Puerto Rico there were approximately 1806 positive cases reported, with a mortality rate ranging among 5% [2] But, is this number even real? At the moment, we are using the Reverse Transcription-PCR for diagnosis;but is this study completely reliable? CASE PRESENTATION: We present a 69-year-old man with multiple chronic comorbidities such as: hypertension, dyslipidemia, diabetes mellitus, chronic kidney disease, bronchial asthma and sleep apnea Patient complained of three days of cough, anorexia, generalized malaise, fever and chills prior to a sudden loss of consciousness episode in which he had a right side 8th rib fracture developing a small apical right sided pneumothorax Laboratory markers such as Ferritin, LDH, sed rate, CRP, D-dimer, lactic acid and procalcitonin, all of which have been associated with COVID-19, were elevated upon admission Chest CT scan demonstrated evidence of the small right apical pneumothorax and a focal consolidation of a left lung base In light of patient’s signs and symptoms, he was considered a person under investigation (PUI) for COVID-19 A nasopharyngeal (NP) RT-PCR was done, yielding a negative test Patient continued developing fever episodes and worsening hypoxemia and his inflammatory markers continued on an increasing trend Chest CT scan was repeated and there was evidence of worsening, bilateral and bibasilar patchy ground glass opacities Another 2 NP RT-PCRs were done in light of high suspicion for COVID-19 pneumonia;both of them came back negative Was there any other etiology that we were missing? Patient was taken to bronchoscopy and the RT-PCR testing for SARS-CoV-2 in BAL came back positive, despite 3 previous negative NP tests results DISCUSSION: RT-PCR is the most useful diagnostic test currently used for the detection of COVID-19 Mostly the nasopharyngeal test is done, mainly due to its simplicity There are multiple existing RT-PCR assays in the market along with several manufacturers [1,3] Among the different assays there are: RT-PCR assays targeting the RNA-dependent RNA polymerase (RdRp), envelope (E), and nucleocapsid (N) genes of SARS-CoV-2;being the most sensitive the RdRp (3) Yi Wei Tang et al recently published a study in which they developed three different RT-PCR assays and compared them to the currently existing and mostly used assay (RdRp) They discovered that the COVID-19-RdRp/Hel assay was the most sensitive and specific of them and the one with less cross-reactivity with other human coronaviruses CONCLUSIONS: This case brings a very important question, how reliable are the currently used RT-PCR tests regarding our Novel Coronavirus? Reference #1: Chan JF-W, et al 2020 Improved molecular diagnosis of COVID- 19 by the novel, highly sensitive and specific COVID-19- RdRp/Hel real-time reverse transcription-PCR assay validated in vitro and with clinical specimens J Clin Microbiol 58:e00310-20 https://doi org/10 1128/JCM 00310-20 Reference #2: https://www nytimes com/interactive/2020/us/puerto-rico-coronavirus-cases html Reference #3: Yi-Wei Tang, et al 2020 The Laboratory Diagnosis of COVID-19 Infection: Current Issues and Challenges J Clin Microbiol Online Edition doi:10 1128/JCM 00512-20 DISCLOSURES: No relevant relationships by Edgardo Adorno, source=Web Response No relevant relationships by Christian Castillo Latorre, source=Web Response No relevant relationships by Francisco Del Olmo-Arroyo, source=Web Response No relevant relationships by Ilean Lamboy, source=Web Response No relevant relationships by Mariana Mercader-Pérez, source=Web Response No relevant relationships by William Rodriguez-Cintron, source=Web Response No relevant relationships by Mark Vergara-Gomez, sour e=Web Response
is ?:annotates of	<https://research.tib.eu/covid-19/entity/yrgpy3zi_hasAnnotation_C5203676>
?:creator	<https://research.tib.eu/covid-19/entity/Lamboy%2C_I.%3B_Rodriguez-Cintron%2C_W.%3B_Adorno%2C_E.%3B_Vergara-Gomez%2C_M.%3B_Del_Olmo-Arroyo%2C_F.%3B_Latorre%2C_C._C.%3B_Mercader-P%C3%A9rez%2C_M.>
?:journal	Chest
?:license	unk
?:publication_isRelatedTo_Disease	<https://research.tib.eu/covid-19/entity/COVID-19>
is ?:relation_isRelatedTo_publication of	<https://research.tib.eu/covid-19/entity/yrgpy3zi_HAS_C5203676_CAUSES_C0032285>
?:source	WHO
?:title	Role of Bronchoscopy in the Diagnosis of Sars-cov-2: Case Report
?:type	<https://research.tib.eu/covid-19/vocab/Publication>
?:who_covidence_id	#860872
?:year	2020

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Value

?:abstract

SESSION TITLE: Global Case Report Posters SESSION TYPE: Global Case Reports PRESENTED ON: October 18-21, 2020 INTRODUCTION: On December 2019 in Wuhan China there were a cluster of cases of an unknown etiology pneumonia, that we later discovered it was caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) [1] In May 2020, in Puerto Rico there were approximately 1806 positive cases reported, with a mortality rate ranging among 5% [2] But, is this number even real? At the moment, we are using the Reverse Transcription-PCR for diagnosis;but is this study completely reliable? CASE PRESENTATION: We present a 69-year-old man with multiple chronic comorbidities such as: hypertension, dyslipidemia, diabetes mellitus, chronic kidney disease, bronchial asthma and sleep apnea Patient complained of three days of cough, anorexia, generalized malaise, fever and chills prior to a sudden loss of consciousness episode in which he had a right side 8th rib fracture developing a small apical right sided pneumothorax Laboratory markers such as Ferritin, LDH, sed rate, CRP, D-dimer, lactic acid and procalcitonin, all of which have been associated with COVID-19, were elevated upon admission Chest CT scan demonstrated evidence of the small right apical pneumothorax and a focal consolidation of a left lung base In light of patient’s signs and symptoms, he was considered a person under investigation (PUI) for COVID-19 A nasopharyngeal (NP) RT-PCR was done, yielding a negative test Patient continued developing fever episodes and worsening hypoxemia and his inflammatory markers continued on an increasing trend Chest CT scan was repeated and there was evidence of worsening, bilateral and bibasilar patchy ground glass opacities Another 2 NP RT-PCRs were done in light of high suspicion for COVID-19 pneumonia;both of them came back negative Was there any other etiology that we were missing? Patient was taken to bronchoscopy and the RT-PCR testing for SARS-CoV-2 in BAL came back positive, despite 3 previous negative NP tests results DISCUSSION: RT-PCR is the most useful diagnostic test currently used for the detection of COVID-19 Mostly the nasopharyngeal test is done, mainly due to its simplicity There are multiple existing RT-PCR assays in the market along with several manufacturers [1,3] Among the different assays there are: RT-PCR assays targeting the RNA-dependent RNA polymerase (RdRp), envelope (E), and nucleocapsid (N) genes of SARS-CoV-2;being the most sensitive the RdRp (3) Yi Wei Tang et al recently published a study in which they developed three different RT-PCR assays and compared them to the currently existing and mostly used assay (RdRp) They discovered that the COVID-19-RdRp/Hel assay was the most sensitive and specific of them and the one with less cross-reactivity with other human coronaviruses CONCLUSIONS: This case brings a very important question, how reliable are the currently used RT-PCR tests regarding our Novel Coronavirus? Reference #1: Chan JF-W, et al 2020 Improved molecular diagnosis of COVID- 19 by the novel, highly sensitive and specific COVID-19- RdRp/Hel real-time reverse transcription-PCR assay validated in vitro and with clinical specimens J Clin Microbiol 58:e00310-20 https://doi org/10 1128/JCM 00310-20 Reference #2: https://www nytimes com/interactive/2020/us/puerto-rico-coronavirus-cases html Reference #3: Yi-Wei Tang, et al 2020 The Laboratory Diagnosis of COVID-19 Infection: Current Issues and Challenges J Clin Microbiol Online Edition doi:10 1128/JCM 00512-20 DISCLOSURES: No relevant relationships by Edgardo Adorno, source=Web Response No relevant relationships by Christian Castillo Latorre, source=Web Response No relevant relationships by Francisco Del Olmo-Arroyo, source=Web Response No relevant relationships by Ilean Lamboy, source=Web Response No relevant relationships by Mariana Mercader-Pérez, source=Web Response No relevant relationships by William Rodriguez-Cintron, source=Web Response No relevant relationships by Mark Vergara-Gomez, sour e=Web Response

is ?:annotates of