PropertyValue
?:abstract
  • Commonly used RT-qPCR-based SARS-CoV-2 diagnostics require 2–3 separate reactions or rely on detection of a single viral target, adding time and cost or risk of false-negative results. Currently, no test combines detection of widely used SARS-CoV-2 E- and N-gene targets and a sample control in a single, multiplexed reaction. We developed the IGI-LuNER RT-qPCR assay using the Luna Probe Universal One-Step RT-qPCR master mix with publicly available primers and probes to detect SARS-CoV-2 N gene, E gene, and human RNase P (NER). This combined, cost-effective test can be performed in 384-well plates with detection sensitivity suitable for clinical reporting, and will aid in future sample pooling efforts, thus improving throughput of SARS-CoV-2 detection.
is ?:annotates of
?:creator
?:doi
?:doi
  • 10.1101/2020.12.10.20247338
?:journal
  • medRxiv
?:license
  • cc-by-nd
?:pdf_json_files
  • document_parses/pdf_json/2114cc59506206d3c79678444158cdc51c950e51.json; document_parses/pdf_json/aa9f31a94f03159cbadf76a3141603809af0d0f5.json
?:pmc_json_files
  • document_parses/pmc_json/PMC7743092.xml.json
?:pmcid
?:pmid
?:pmid
  • 33330883.0
?:publication_isRelatedTo_Disease
?:sha_id
?:source
  • MedRxiv; Medline; PMC; WHO
?:title
  • IGI-LuNER: single-well multiplexed RT-qPCR test for SARS-CoV-2
?:type
?:year
  • 2020-12-11

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