PropertyValue
?:abstract
  • To explore genomic characteriation of 2019-nCoV, throat swab specimens from COVID-19 patients were inoculated on Vero-E6 cells Six days post-inoculation, supernatants of cell cultures were collected, tested, and sequenced on the Ion Torrent S5 Next-Generation Sequencing system Two strains viruses were isolated from 12 specimens (eight of them were positive in ORF1ab gene and N gene, one of them was positive in N gene and three of them were negative in ORF1ab gene and N gene), as confirmed by online BLAST search at NCBI website Genetic similarity of more than 99 9% were observed, from genomic level to structure genes including E, M, N and S genes, between the 2 isolates and the 2019-nCoV reference strain Wuhan-Hu-1 that isolated from Wuhan, Hubei province Therefore, these results indicated that 2019-nCoV isolates in Fujian province have not mutated significantly yet so far
is ?:annotates of
?:creator
?:journal
  • Chinese_Journal_of_Zoonoses
?:license
  • unk
?:publication_isRelatedTo_Disease
?:source
  • WHO
?:title
  • Isolation and genome sequence analyses of 2019-nCoV from COVID-19 patients in Fujian Province, China
?:type
?:who_covidence_id
  • #833456
?:year
  • 2020

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